Q1.
The DNA molecule to which the gene of interest is integrated for cloning is called
Q2.
The cutting of DNA at specific locations became possible with the discovery of
Q3.
Which one of the following is a case of wrong matching?
Q4.
Which one of the following techniques made it possible to genetically engineer living organisms?
Q5.
Which of the following are used in gene cloning?
Q6.
The bacteria generally used for genetic engineering is
Q7.
Which of the following is related to genetic engineering?
Q8.
Which of the following organelles is related with genetic engineering?
Q9.
Identify the wrong statement with regard to restriction enzymes.
Q10.
Choose the correct pair from the following.
Q11.
The specific palindromic sequence which is recognised by EcoRI is
Q12.
The sequence that controls the copy number of the linked DNA in the vector, is termed
Q13.
In gel electrophoresis, separated DNA fragments can be visualized with the help of
Q14.
Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.
Q15.
A selectable marker is used to
Q16.
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
Q17.
The DNA fragments separated on an agarose gel can be visualised after staining with
Q19.
A gene whose expression helps to identify transformed cell is known as
Q20.
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
Q21.
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
Q22.
Which of the following restriction enzymes produces blunt ends?
Q23.
Which of the following is not a feature of the plasmids?
Q24.
Which of the following is a restriction endonuclease?
Q25.
The introduction of T-DNA into plants involves
Q26.
Which vector can clone only a small fragment of DNA?
Q27.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Q28.
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
Q29.
A single strand of nucleic acid tagged with a radioactive molecule is called
Q30.
For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of
Q31.
Biolistics (gene-gun) is suitable for
Q32.
Which one of the following represents a palindromic sequence in DNA?
Q33.
Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′
Q34.
There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?
Q35.
Agarose extracted from sea weeds is used in
Q36.
Which one of the following is used as vector for cloning genes into higher organisms?
Q37.
DNA or RNA segment tagged with a radioactive molecule is called
Q38.
Restriction endonucleases are enzymes which
Q39.
In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors.
(i) Bacterium (ii) Plasmid
(iii) Plasmodium (iv) Bacteriophage
Q40.
Polyethylene glycol method is used for
Q41.
Which one of the following is commonly used in transfer of foreign DNA into crop plants?
Q42.
Gel electrophoresis is used for
Q43.
The linking of antibiotic resistance gene with the plasmid vector became possible with
Q44.
Two microbes found to be very useful in genetic engineering are
Q45.
The Ti plasmid, is often used for making transgenic plants. The plasmid is found in
Q46.
Which of the following enzymes are used to join bits of DNA?
Q47.
Which of the following cut the DNA from specific places?
Q48.
Plasmid has been used as vector because
Q49.
Two bacteria found to be very useful in genetic engineering experiments are
Q50.
Match the following? a,b,c,d respectively
| Organism |
Use |
| (a) Bacillus thuringiensis |
(iv) Cry proteins |
| (b) Thermus aquaticus |
(iii) DNA polymerase |
| (c) Agrobacterium tumefaciens |
(i) Cloning vector |
| (d) Salmonella typhimurium |
(ii) Construction of first rDNA |
Q51.
DNA precipitation out of a mixture of biomolecules can be achieved by treatment with
Q52.
Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?
Q53.
The correct order of steps in Polymerase Chain Reaction (PCR) is
Q54.
The process of separation and purification of expressed protein before marketing is called
Q55.
Stirred-tank bioreactors have been designed for
Q56.
Which of the following is not a component of downstream processing?
Q57.
The Taq polymerase enzyme is obtained from
Q58.
PCR and restriction fragment length polymorphism are the methods for
Q59.
Which one is a true statement regarding DNA polymerase used in PCR?
Q60.
During the process of isolation of DNA, chilled ethanol is added to
Q61.
Stirred-tank bioreactors have been designed for